Contractile Proteins Content of Long Term Permanent Denervated Human Muscle after Functional Electrical Stimulation
نویسندگان
چکیده
Severe atrophy of skeletal muscles occurs during long-term permanent denervation (LTPD). We studied 14 human muscle biopsies taken from patients suffering lower motoneuron denervation from 2 to 30 years. Patients were treated with Functional Electrical Stimulation (FES) for different periods of time (0.5 to 6 years). We describe new gel electrophoresis analyses, which allow to quantify protein markers of damage/degeneration and recovery/regeneration of muscle tissue. Electrical stimulation of denervated muscles increases size of the myofibres, maintains sarcomeres preventing/reversing apoptosis/necrosis and secondary degeneration of the long-term denervated tissue. In this work we quantify myosin content in long-term permanent denervated muscles without and with FES. Results confirm the effectiveness of the FES training.
منابع مشابه
Permanent denervation of rat Tibialis Anterior after bilateral sciatectomy: Determination of chronaxie by surface electrode stimulation during progression of atrophy up to one year
Permanent lack of motor innervation results in striking alterations of functional and structural properties of muscle. In SCI patients, direct Functional Electrical Stimulation of denervated muscles (FES of DDM) prevents or reverts muscle atrophy. FES is, however, believed to be effective when started early after SCI, since long term denervated muscle do not contract by commercial surface elect...
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Following denervation, skeletal muscle undergoes rapid loss in both mass and contractile force, with an accompanying series of changes in structure, biochemistry and physiology. Morphologic features of the long-term denervated muscle suggest that the original fibers are lost and those seen are the results of repeated cycles of cell death and regeneration. Markers of myogenic events in adult mus...
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